Commercial-scale mushroom cultivation

This is an example of a commercial-scale straw culture operation:

Basic Steps

1. Obtain mother culture and keep refrigerated
2. Replicate small piece of mother culture in petri dishes with agar
3. Sterilize barley or other grain in a pressure cooker
4. Mix culture from petri dishes into sterilized grain in bags kept in a sterile, temperature regulated environment
5. Sterilize straw in steam boiler room (or other method)
6. Mix 1:4 inoculated grain with straw in bags